The Histology Guide

Male Reproductive System: Spermatogenesis

The production of sperm and eggs/ova (gametes) is a procedure called gametogenesis (spermatogenesis and oogenesis). Gametogenesis involves two rounds of meiotic cell division, in which one diploid cell gives rise to 4 haploid cells.

This diagram shows the processes involved in spermatogenesis. The germinal (seminiferous epithelium) of the seminiferous tubules contains spermatogenic cells and Sertoli cells. (Click here to find out about Sertoli cells)

The spermatogenic cells divide by mitosis, then meiosis to form gametes, which mature into sperm by the process of spermiogenesis. Unusually, the developing spermatogenic cells remain connected by cytoplasmic bridges, until they have formed a mature spermatozoan.

There are three types of spermatogonia -

It takes about 2 months for pale A spermatogonia to form new spermatozoa, the total production time for mature human spermatozoa is 74 days.

Compare this process with oogenesis:

In spermatogenesis, each of the four gametes produced by meiosis develops into a mature spermatozoan. What happens to the four gametes in females? (click here to remind yourself).

In spermatogenesis, primitive germ cells are only present in small numbers before puberty. After puberty (driven by testosterone) the spermatogonia multiply continuously to form male gametes. What happens in females?

Seminiferous Tubule

This photograph shows a single seminiferous tubule, (as found after puberty) at high magnification.

The 50µmseminiferous tubule is lined by a complex stratified epithelium, lined by a basal membrane and covered by a sheath of connective tissue. The connective tissue contains fibroblasts and myoid cells. The myoid cels are contractile, and produce gentle peristaltic waves in the tubules.

The Sertoli cells have pale nuclei and dense nucleoli. The spermatogonia and the nuclei of the Sertoli cells lie on the basal membrane. The nuclei of these cells are pale, with prominent nuclolei.

Primary spermatocytes, secondary spermatocytes, spermatids and spermatozoa are in the adluminal compartment.

The lumen also contains some spermatozoa, and cellular debris.

Using a textbook to help you see if you can identify the features listed above on the photograph.

This schematic diagram shows the arrangement of Sertoli cells and developing spermatocytes in the seminiferous tubules.

More information about Sertoli cells and Leydig cells.